Home > Sandwich ELISA Kits > Mouse Runt Related Transcription Factor 3 (RUNX3) ELISA Kit

Product Name:

Mouse Runt Related Transcription Factor 3 (RUNX3) ELISA Kit

Cat.#:

JL54455

Regulatory Status:

RUO

Alternative:

AML2; CBFA3; PEBP2aC; PEBP2A3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; Polyomavirus enhancer-binding protein 2 alpha C subunit

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.083ng/mL

Specificity:

This kit recognizes Mouse RUNX3 in samples. No significant cross-reactivity or interference between Mouse RUNX3 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates;cell culture supernatants and other biological fluids

Assay Procedure:

Research Area:

Developmental Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| RUNX3

Runt Related Transcription Factor 3 (RUNX3) is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters,and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.In melanocytic cells RUNX3 gene expression may be regulated by MITF.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse RUNX3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	93
Cell Lysates(n=8)	95-105	102

| Linearity

Samples were spiked with high concentrations of Mouse RUNX3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98